Colorimetric recognition of pazufloxacin mesilate based on the aggregation of gold nanoparticles

• A platform for optical detection pazufloxacin mesilate was established through its interaction with AuNPs.
• Different concentrations of pazufloxacin mesilate could be determined with naked eye or UV–vis spectrophotometer.
• This method owned low detection limit and high selectivity.
• This proposed method was applied to analysis of pazufloxacin mesilate in human urine samples.

A novel colorimetric nanomaterial-assisted optical sensor for pazufloxacin mesilate was proposed for the first time. Pazufloxacin mesilate could induce the aggregation of glucose-reduced gold nanoparticles (AuNPs) through hydrogen-bonding interaction and electrostatic attraction, leading to the changes in color and absorption spectra of AuNPs. The effect of different factors such as pH, the amount of AuNPs, reaction time and reaction temperature was inspected. Under the optimum condition, UV–vis spectra showed that the absorption ratio (A670/A532) was linear with the concentration of pazufloxacin mesilate in the range from 9 × 10− 8 mol L− 1 to 7 × 10− 7 mol L− 1 with a linear coefficient of 0.9951. This method can be applied to detecting pazufloxacin mesilate with an ultralow detection limit of 7.92 × 10− 9 mol L− 1 without any complicated instruments. Through inspecting other analytes and ions, the anti-interference performance of AuNP detection system for pazufloxacin mesilate was excellent. For its high efficiency, rapid response rate as well as wide linear range, it had been successfully used to the analysis of pazufloxacin mesilate in human urine quantificationally.

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