Development of a novel pH sensor based upon Janus Green B immobilized on triacetyl cellulose membrane: Experimental design and optimization

• High selectivity and sensitivity pH optical sensitive sensor for Janus Green B dye
• Box–Behnken design for optimization of conditions
• On-line, no leakage, long-term stability and fast response time

A novel pH optical sensor was prepared by immobilizing an azo dye called Janus Green B on the triacetylcellulose membrane. Condition of the dye solution used in the immobilization step, including concentration of the dye, pH, and duration were considered and optimized using the Box–Behnken design. The proposed sensor showed good behavior and precision (RSD < 5%) in the pH range of 2.0–10.0. Advantages of this optical sensor include on-line applicability, no leakage, long-term stability (more than 6 months), fast response time (less than 1 min), high selectivity and sensitivity as well as good reversibility and reproducibility.

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