Mixed micelle cloud point-magnetic dispersive μ-solid phase extraction of doxazosin and alfuzosin

• Mixed micelle cloud point extraction combined with magnetic dispersive μ-solid phase extraction.
• The method significant simplification to conventional MM-CPE procedures.
• The limits of detection were 0.21 and 0.16 ng mL−1 for DOX and ALF, respectively.
• Successfully applied for the determination of the drugs in tablets, urine, and plasma.

Mixed micelle cloud point extraction (MM-CPE) combined with magnetic dispersive μ-solid phase extraction (MD-μ-SPE) has been developed as a new approach for the extraction of doxazosin (DOX) and alfuzosin (ALF) prior to fluorescence analysis. The mixed micelle anionic surfactant sodium dodecyl sulfate and non-ionic polyoxyethylene(7.5)nonylphenylether was used as the extraction solvent in MM-CPE, and diatomite bonding Fe3O4 magnetic nanoparticles were used as the adsorbent in MD-μ-SPE. The method was based on MM-CPE of DOX and ALF in the surfactant-rich phase. Magnetic materials were used to retrieve the surfactant-rich phase, which easily separated from the aqueous phase under magnetic field. At optimum conditions, a linear relationship between DOX and ALF was obtained in the range of 5–300 ng mL−1, and the limits of detection were 0.21 and 0.16 ng mL−1, respectively. The proposed method was successfully applied for the determination of the drugs in pharmaceutical preparations, urine samples, and plasma samples.

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