کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1230079 | 1495218 | 2015 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification](/preview/png/1230079.png)
• A label-free ECL aptasensor for sensitive detection of IgE was developed.
• Petal-like CdS nanospheres were prepared and utilized as ECL indicator.
• AuNPs co-immobilized with CdS enhanced the ECL intensity.
• The method was applied for the determination of IgE in human serum sample.
A facile label-free electrochemiluminescence (ECL) aptasensor was designed for sensitive detection of human immunoglobulin E (IgE) using petal-like CdS nanospheres and Au nanoparticles (AuNPs) as sensing platform. To construct the aptasensor, petal-like CdS nanospheres as ECL emitter were firstly synthesized and immobilized on the chitosan-coated glassy carbon electrode (GCE) surface. Chitosan was coated on the CdS/CS/GCE again with two coating numbers to produce a stable ECL signal and facilitate subsequent AuNPs immobilization. The construction of aptasensor was achieved after IgE aptamer was adsorbed onto the AuNPs. The detection of IgE was performed upon the incubation of the interface with target protein IgE. Under the optimum conditions, the ECL signal decreased depending linearly on the logarithmic value of IgE concentration ranging from 5.0 × 10−13 to 1.0 × 10−9 M with a regression equation of I = −15254.8 − 2129.3 log c (R2 = 0.996). The detection limit was experimentally found to be 8.0 × 10−14 M. The applicability of the constructed aptasensor was demonstrated in the determination of IgE in human serum samples.
Figure optionsDownload as PowerPoint slide
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 151, 5 December 2015, Pages 274–279