Simultaneous spectrophotometric determination of Celecoxib and Diacerein in bulk and capsule by absorption correction method and chemometric methods

• Development and validation of chemometric methods for simultaneous estimation of DIA and CEL.
• Development and validation of absorption correction method for simultaneous estimation of DIA and CEL.
• Statistical comparison of developed methods using ANOVA.

Two methods, absorption correction and multivariate spectrophotometric methods were developed for simultaneous estimation of Celecoxib (CEL) and Diacerein (DIA) in combined dosage form. Absorption correction method involves direct estimation of DIA at wavelength 341 nm in which CEL has zero absorbance and shows no interference. For estimation of CEL, corrected absorbance was calculated at 253 nm due to the interference of DIA at this wavelength. Linearity was observed in the range of 6–22 μg mL−1 for CEL and 3–11 μg mL−1 for DIA. The method was validated as per ICH guidelines. Chemometric methods including classical least square (CLS), inverse least square (ILS), principal component regression (PCR) and partial least square (PLS) were studied for simultaneous determination of CEL and DIA in capsule using spectrophotometry. A set of 25 standard mixtures containing both drugs were prepared in range of 5–25 μg mL−1 for CEL and 3–15 μg mL−1 for DIA. Analytical figure of merit (FOM), such as sensitivity, selectivity, analytical sensitivity, limit of detection and limit of quantitation were determined for chemometric methods. The proposed methods were applied for determination of two components from combined dosage form.

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