کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1230161 | 1495243 | 2014 | 6 صفحه PDF | دانلود رایگان |
• DHA and 9-OH DHA both induce the heme group of BHb.
• DHA and 9-OH DHA both can change the polarity of BHb.
• DHA and 9-OH DHA both make BHb unfolding.
• The interaction between BHb and DHA is stronger than 9-OH DHA.
The UV–vis absorption, steady state/time resolved fluorescence spectroscopy and synchronous fluorescence, circular dichroism (CD) spectroscopy are used to investigate the interaction mechanisms of dihydroartemisinin (DHA) and 9-hydroxy-dihydroartemisinin (9-OH DHA), respectively. The UV–vis studies present that DHA and 9-OH DHA can disturb the structure of bovine hemoglobin (BHb). Steady state/time resolved and synchronous fluorescence spectroscopy reveal that the binding constant of DHA with BHb is bigger than 9-OH DHA. CD spectra indicate DHA and 9-OH DHA can change the conformation of BHb. The comparison results suggest that the binding of BHb with DHA is more stable and stronger than 9-OH DHA.
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Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 125, 5 May 2014, Pages 120–125