Cyclodextrin polymer/Fe3O4 nanocomposites as solid phase extraction material coupled with UV–vis spectrometry for the analysis of rutin

• A novel solid phase extraction material coupled with UV–vis spectrometry for the analysis of rutin.
• CM-HP-β-CD polymer modified Fe3O4 nanoparticles (CM-HP-β-CDCP-MNPs) were synthesized/characterized.
• The adsorption of rutin onto CM-HP-β-CDCP-MNPs was rapid, quantitative.
• This technique was successfully applied to determine rutin in real samples.

In this paper, carboxymethyl-hydroxypropyl-β-cyclodextrin polymer modified magnetic particles Fe3O4 (CM-HP-β-CDCP-MNPs) were prepared and applied to magnetic solid phase extraction of rutin combined with UV–visible spectrometry detection. The synthesized magnetic particles were characterized by element analysis, Fourier transform infrared spectra, thermal gravimetric analysis, and transmission electron microscopy. Several variables affecting the extraction and desorption of rutin such as pH, the amount of adsorbent, the type and volume of eluent, extraction and desorption time, and temperature were investigated. The maximum adsorption capacity was 67.0 mg g−1 for rutin with the equilibrium time of 30 min at room temperature, and the adsorbent could be reused for 10 times. A calibration curve was linear in the range of 0.05–8.00 μg mL−1 with a relative standard deviation of 2.9% (n = 5, c = 4.0 μg mL−1). The limit of detection was 7.0 ng mL−1. The interaction mechanism between the adsorbent and rutin was also studied. Feasibility of this method was validated by the analysis of rutin tablets and lotus plumule.

The adsorption efficiencies of rutin with CM-HP-β-CDCP-MNPs as sorbent (curve a) was much higher than that with Fe3O4 (curve b) in the tested pH range.Figure optionsDownload as PowerPoint slide