The detection for hypochlorite by UV–Vis and fluorescent spectra based on oxidized ring opening and successive hydrolysis reaction

• Two new fluorescent probes for hypochlorite were developed.
• The detection could be realized in quasi-aqueous phase with low detection.
• The probe could be used to monitor intracellular ClO− in HepG2 cells.

In this work, two high selective and sensitive fluorescent probes for ClO−, 7-Hydroxycoumarin and 4-Hydroxycoumarin were designed. The reaction mechanism that we speculated was the oxidized ring opening reaction and hydrolysis. The detection could be realized in quasi-aqueous phase and the detection limits of probe [7] and probe [4] for ClO− were found to be 56.8 nM and 70.5 nM. Furthermore, the probes can be used to cell imagings.

Figure optionsDownload as PowerPoint slide