Anti-proliferative effect of a compound isolated from Cassia auriculata against human colon cancer cell line HCT 15

• A compound was isolated for the first time from leaves of Cassia auriculata.
• The isolated compound was structurally identified by HPLC, LC–MS, UV–vis, FTIR and NMR.
• This compound had potential to prevent colon cancer cell line HCT 15.
• Isolated compound depicted anti-proliferative effect against colon cancer.

The compound was isolated from leaves of Cassia auriculata and its structure was characterized using high-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LC–MS), UV–vis spectroscopy (UV–vis), fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, cytotoxicity, nuclear morphology and lactate dehydrogenase assay of isolated compound was tested against human colon cancer cell line HCT 15. The isolated compound, 4-(4-chlorobenzyl)-2,3,4,5,6,7-hexahydro-7-(2-ethoxyphenyl)benzo[h][1,4,7]triazecin-8(1H)-one at 25 μg/ml concentration and by 48 h showed 50% inhibition of human colon cancer cells (HCT 15). The results suggest that isolated compound from C. auriculata has potential to prevent colon cancer cell line.

Figure optionsDownload as PowerPoint slide