Evaluation of the efficiency of continuous wavelet transform as processing and preprocessing algorithm for resolution of overlapped signals in univariate and multivariate regression analyses; an application to ternary and quaternary mixtures

• Quantification of Drotaverine, Caffeine, Paracetamol and Para aminophenol using accurate methods that could be applied in QC labs.
• Environment friendly univariate and multivariate methods were applied validated as per ICH.
• The methods can be applied for the analysis of complex mixtures without preliminary separation.
• CWT as processing or pre-processing method for spectral signals preceding PLS algorithm.

Wavelets have been adapted for a vast number of signal-processing applications due to the amount of information that can be extracted from a signal. In this work, a comparative study on the efficiency of continuous wavelet transform (CWT) as a signal processing tool in univariate regression and a pre-processing tool in multivariate analysis using partial least square (CWT-PLS) was conducted. These were applied to complex spectral signals of ternary and quaternary mixtures. CWT-PLS method succeeded in the simultaneous determination of a quaternary mixture of drotaverine (DRO), caffeine (CAF), paracetamol (PAR) and p-aminophenol (PAP, the major impurity of paracetamol). While, the univariate CWT failed to simultaneously determine the quaternary mixture components and was able to determine only PAR and PAP, the ternary mixtures of DRO, CAF, and PAR and CAF, PAR, and PAP. During the calculations of CWT, different wavelet families were tested. The univariate CWT method was validated according to the ICH guidelines. While for the development of the CWT-PLS model a calibration set was prepared by means of an orthogonal experimental design and their absorption spectra were recorded and processed by CWT. The CWT-PLS model was constructed by regression between the wavelet coefficients and concentration matrices and validation was performed by both cross validation and external validation sets. Both methods were successfully applied for determination of the studied drugs in pharmaceutical formulations.

First coeff. of CWL curves of 9 μg/mL DRO (), 5 μg/mL CAF () and 9 μg/mL PAR ().Figure optionsDownload as PowerPoint slideFigure optionsDownload as PowerPoint slideFigure optionsDownload as PowerPoint slideFigure optionsDownload as PowerPoint slide