A sensitive and selective resonance Rayleigh scattering method for quick detection of avidin using affinity labeling Au nanoparticles

• Resonance rayleigh scattering method to detect avidin using affinity Au nanoparticles was studied.
• This system was quick and sensitive for avidin detection due to avidin–biotin affinitive interaction.
• Resonance rayleigh scattering intensity of biotin–AuNPs increase linearly with the increasing concentration of avidin.
• This method was applied to quantitative determination of the avidin in synthetic samples and egg products with stable recovery and RSD value.

Avidin is a glycoprotein with antinutritional property, which should be limited in daily food. We developed an affinity biosensor system based on resonance Rayleigh scattering (RRS) and using affinity biotin labeling Au nanoparticles (AuNPs). This method was selective and sensitive for quick avidin detection due to the avidin–biotin affinitive interaction. Under optimal conditions, RRS intensity of biotin–AuNPs increase linearly with an increasing concentration of avidin from 5 to 160 ng/mL. The lower limit of detection was 0.59 ng/mL. This rapid and selective avidin detection method was used in synthetic samples and egg products with recoveries of between 102.97 and 107.92%, thereby demonstrating the feasible and practical application of this assay.

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