Understanding the stability of silver nanoparticles bio-fabricated using Acacia arabica (Babool gum) and its hostile effect on microorganisms

• Green route synthesis of silver nanoparticles (SNPs) of ∼35 nm diameter using Acacia arabica gum extract.
• SNPs were highly stable till 4 M NaCl concentration.
• Flocculation parameter of SNPs was calculated.
• Sliver nanoparticles were anti-microbial against Bacillus subtilis and Pseudomonas aeruginosa.

We report green synthesis of stable silver nanoparticles (SNPs) from Acacia arabica gum and its anti-bacterial activity against gram-positive and gram-negative bacteria. UV–Vis spectral analysis of synthesized SNPs showed maximum peak at 462 nm initially and 435 nm after 24 h. Using Transmission Electron microscopy (TEM), the average size of synthesised SNPs was found to be ∼35 nm. X-ray diffraction (XRD) and Selective area electron diffraction (SAED) pattern confirmed the crystalline nature of SNPs. Percentage conversion of Ag+ ions into Ag° was calculated using ICP-AES and was found to be 94%. By calculating flocculation parameter, we could see that these SNPs are extremely stable under the influence of very high NaCl concentration up to 4.16 M. These stable SNPs can be used in various industrial and medical applications.

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