Determination of captopril by high-performance liquid chromatography with direct electrogenerated chemiluminescence

Captopril exhibit electrogenerated chemiluminescence (ECL) in NaNO3 solution when constant current is exerted. Based on this observation, a direct ECL method coupled with high-performance liquid chromatography (HPLC) separation is developed for determination of captopril in human serum. Factors affected the ECL emission are investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of captopril in the range of 4.0 × 10−6–2.0 × 10−3 g mL−1 and the detection limit is 2 × 10−6 g mL−1 (S/N = 3). Compared with the common electrogenerated chemiluminescence experiments, the developed method need no any other fluorescence additives.

Figure optionsDownload as PowerPoint slideHighlights
► Captopril exhibit electrogenerated chemiluminescence (ECL) in NaNO3 solution.
► ECL without other fluorescence additives.
► Active oxygen reacted with captopril to produce ECL emission.