کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1232037 1495265 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A comparison study on the binding of hesperetin and luteolin to bovine serum albumin by spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A comparison study on the binding of hesperetin and luteolin to bovine serum albumin by spectroscopy
چکیده انگلیسی

Binding mechanism of luteolin (LUT) and hesperetin (HES) to bovine serum albumin (BSA) was investigated at 288,298,310 K and pH = 7.40 by UV absorption spectroscopy, fluorescence quenching and synchronous fluorescence spectroscopy. Under simulated physiological conditions, the fluorescence data indicated that hesperetin binding to BSA mainly occurs through a static mechanism. In contrast, binding of luteolin to BSA is a combined quenching process while static quenching is prevailing. Linear interval of the Stern–Volmer plot of LUT–BSA for the concentration ratio of LUT to BSA ranged from 0.5 to 1.25 was obtained. The thermodynamic parameters obtained from the Van’t Hoff equation indicated that electrostatic force was the predominant force in the LUT–BSA and HES–BSA complex. The inner filter effect was eliminated to get accurate data. The conformational changes of BSA caused by LUT and HES were observed in the UV absorption. Results of fluorescence quenching and synchronous fluorescence showed that degree of luteolin–BSA quenching was higher than hesperetin–BSA quenching, which indicated that the 4′-hydroxide radical was more helpful to the ligand binding to proteins than 4′-methoxyl group for flavones.

Figure optionsDownload as PowerPoint slideHighlights
► Binding of hesperetin to BSA is typical static quenching.
► Luteolin binds to BSA as a combined quenching and static quenching is prevailing.
► A SV plot linear interval of luteolin binding to BSA ranges from 0.5 to 1.25.
► 4′-Hydroxide group is more helpful for flavone binding to BSA than 4′-methoxyl group.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 103, 15 February 2013, Pages 114–119
نویسندگان
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