Application of the ratio difference spectrophotometry to the determination of ibuprofen and famotidine in their combined dosage form; Comparison with previously published spectrophotometric methods

• Application of the ratio difference method for the determination of ibuprofen and famotidine.
• The proposed method is simpler and does not need tedious mathematical calculations.
• The proposed method is more accurate as it does not depend on measurement at a single wavelength.
• Statistical analysis of the results showed the method is accurate and precise.
• Comparison with the previously published methods.

Ratio difference spectrophotometric method was developed for the determination of ibuprofen and famotidine in their mixture form. Ibuprofen and famotidine were determined in the presence of each other by the ratio difference spectrophotometric (RD) method where linearity was obtained from 50 to 600 μg/mL and 2.5 to 25 μg/mL for ibuprofen and famotidine, respectively. The suggested method was validated according to ICH guidelines and successfully applied for the analysis of ibuprofen and famotidine in their pharmaceutical dosage forms without interference from any additives or excipients.

Ratio spectra of (A) ibuprofen using the spectrum of 2.5 μg/mL famotidine as divisor and (B) famotidine using the spectrum of 300 μg/mL ibuprofen as divisor showing wavelengths at which measurements were done.Figure optionsDownload as PowerPoint slide