On-column enrichment and surface-enhanced Raman scattering detection in nanoparticles functionalized porous capillary monolith

• Au nanoparticles functionalized monolithic column was designed for SERS.
• The designed monolith was applied for on-column enrichment and SERS detection.
• The SERS column provides high SERS sensitivity and good reproducibility.

A monolithic column functionalized with gold nanoparticles (GNPs) was designed to provide ultrasensitive detection with surface-enhanced Raman scattering (SERS). The monolithic column based on poly (glycidyl methacrylate-co-ethylene dimethacrylate) (GMA-co-EDMA) was served as the enrichment sorbent to concentrate GNPs and targets. We found that 60 nm GNPs-functionalized monolithic columns demonstrated the best SERS enhancement, and the lowest detectable concentration for PATP and CV could be achieved at 10−7 and 10−11 M, respectively. Moreover, the columns exhibit a good reproducibility in both spot-to-spot (∼10%) and batch-to-batch (∼15%). The SERS monolithic column with a high sensitivity and reproducibility has a great potential in the field-based rapid detection of targets in complex real-world samples.

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