Application of Savitzky–Golay differentiation filters and Fourier functions to simultaneous determination of cefepime and the co-administered drug, levofloxacin, in spiked human plasma

• SGDF and FF methods > sensitive, accurate, precise, selective.
• Successfully applied in plasma > good > selectivity, accuracy, precision, stability, extraction efficiency.
• No significant statistical difference from the reported methods.

The present work is concerned with simultaneous determination of cefepime (CEF) and the co-administered drug, levofloxacin (LEV), in spiked human plasma by applying a new approach, Savitzky–Golay differentiation filters, and combined trigonometric Fourier functions to their ratio spectra. The different parameters associated with the calculation of Savitzky–Golay and Fourier coefficients were optimized. The proposed methods were validated and applied for determination of the two drugs in laboratory prepared mixtures and spiked human plasma. The results were statistically compared with reported HPLC methods and were found accurate and precise.

Figure optionsDownload as PowerPoint slide