Validated spectrofluorimetric method for determination of selected aminoglycosides

• New spectroflourimetric method for the analysis of selected aminoglycosides.
• The proposed method is successfully applied for the analysis of in different dosage forms and pure powders.
• The method is based on Hantzsch condensation reaction.
• The proposed method can be considered of a great value in quality control analysis of the selected drugs.

New, sensitive, and selective spectrofluorimetric method was developed for determination of three aminoglycoside drugs in different dosage forms, namely; neomycin sulfate (NEO), tobramycin (TOB) and kanamycin sulfate (KAN). The method is based on Hantzsch condensation reaction between the primary amino group of aminoglycosides with acetylacetone and formaldehyde in pH 2.7 yielding highly yellow fluorescent derivatives measured emission (471 nm) and excitation (410 nm) wavelengths. The fluorescence intensity was directly proportional to the concentration over the range 10–60, 40–100 and 5–50 ng/mL for NEO, TOB and KAN respectively. The proposed method was applied successfully for determination of these drugs in their pharmaceutical dosage forms.

Fluorescence spectra of the reaction product of NEO (26 ng mL−1), as a representative example, with acetylacetone and formaldehydeFigure optionsDownload as PowerPoint slide