Sensitive and selective determination of glucose in human serum and urine based on the peroxyoxalate chemiluminescence reaction of a new Fluorophore

A novel method for simple and sensitive determination of glucose based on the peroxyoxalate chemiluminescence (PO-CL) detection of enzymatically generated H2O2 was investigated. Influence of various experimental parameters on glucose sensing, including the action time of the enzyme, solution pH, interferents and the concentration of CL reagents was investigated. Under the optimum condition, the linear response range of glucose was found to be 2.50 × 10−6 to 1.75 × 10−4 mol/L, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) was 1.10 × 10−6 mol/L. The present method has been used to determine the glucose concentrations in real serum and urine samples with satisfactory results.

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► Simple and sensitive determination of glucose.
► Coupling the sensitivity of the CL with the selectivity of the enzymatic reactions.
► Minimum instrumentation requirements.
► Analysis of serum and urine.
► Small sample sizes.