Simultaneous spectrophotometric determination of atrazine and cyanazine by chemometric methods

A spectrophotometric method for the simultaneous determination of two herbicides, atrazine and cyanazine, is described for the first time based on their reaction with p-aminoacetophenone in the presence of pyridine in hydrochloric acid medium. The absorption spectra were measured in the wavelength range of 400–600 nm. The optimized method indicated that individual analytes followed Beer's law in the concentration ranges for atrazine and cyanazine were 0.2–3.5 mg L−1 and 0.3–5.0 mg L−1, and the limits of detection for atrazine and cyanazine were 0.099 and 0.15 mg L−1, respectively. The original and first-derivative absorption spectra of the binary mixtures were performed as a pre-treatment on the calibration matrices prior to the application of chemometric models such as classical least squares (CLS), principal component regression (PCR), partial least squares (PLS). The analytical results obtained by using these chemometric methods were evaluated on the basis of percent relative prediction error and recovery. It was found that the application of PCR and PLS models for first-derivative absorbance data gave the satisfactory results. The proposed methods were successfully applied for the simultaneous determination of the two herbicides in several food samples.