Synthesis, characterization, in vitro anti-proliferative and hemolytic activity of hydroxyapatite

• Nanocrystalline HAP was synthesized by chemical-precipitation method at low temperature.
• The anti-proliferative and hemolytic activity studies of synthesized HAP conforms the anti-cancer activity.
• In vitro anti-proliferative and hemolytic activities of synthesized HAP has mild activity against erythrocytes.

Hydroxyapatite (Ca10(PO4)6(OH)2, HAP) nanoparticles are widely used in several biomedical applications due to its compositional similarities to bone mineral, excellent biocompatibility and bioactivity, osteoconductivity. In this present investigation, HAP nanoparticles synthesized by precipitation technique using calcium nitrate and di-ammonium phosphate. The crystalline nature and the functional group analysis are confirmed using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Fourier transform Raman spectroscopy (FT-Raman) respectively. The morphological observations are ascertained from field emission electron scanning electron microscope (FE-SEM) and transmission electron microscope (TEM). In vitro anti-proliferative and hemolytic activities are carried out on the synthesized HAP samples and the studies reveals that HAP have mild activity against erythrocytes.

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