کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1234267 | 1495244 | 2014 | 6 صفحه PDF | دانلود رایگان |
• Spectroscopic and molecular modeling methods were used.
• The quenching of DNA–EB system by 5-HMF was a static quenching.
• The binding of 5-HMF to DNA was a non- intercalative binding.
• Hydrophobic force and hydrogen bonds both played a major role.
In this work, the interaction of 5-Hydroxymethyl-2-furfural (5-HMF) with calf thymus DNA (ctDNA) under simulated physiological conditions (Tris–HCl buffer of pH 7.40), was explored by UV absorption spectroscopy, fluorescence spectroscopy and molecular modeling method, using ethidium bromide (EB) as a fluorescence probe of DNA. The fluorescence quenching mechanism of EB–ctDNA by 5-HMF was confirmed to be a static quenching, which derived from the formation of a new complex. The binding constants of 5-HMF with DNA in the presence of EB were calculated to be 2.17 × 103, 4.24 × 103 and 6.95 × 103 L mol−1 at 300, 305 and 310 K, respectively. The calculated thermodynamic parameters, enthalpy change ΔH and entropy change ΔS, suggested that both hydrophobic interactions and hydrogen bonds played a predominant role in the binding of 5-HMF to DNA. According to the UV absorption spectroscopy and melting temperature (Tm) curve results, the binding mode of 5-HMF with DNA was indicative of a non-intercalative binding, which was supposed to be a groove binding. The molecular modeling results showed that 5-HMF could bind into the hydrophobic region of ctDNA and supported the conclusions obtained from the above experiments.
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Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 124, 24 April 2014, Pages 78–83