کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1234324 1495244 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New insight into the binding interaction of hydroxylated carbon nanotubes with bovine serum albumin
ترجمه فارسی عنوان
بینش جدیدی در ارتباط متقابل نانولوله های کربنی هیدروکسید شده با آلبومین سرم گاو
کلمات کلیدی
نانولوله های کربنی هیدروکلیک چند سیلند، آلبومین سرم گاو، طیف سنجی فلورسانس، ساز و کار اتصال، تغییرات سازمانی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Binding interaction of HO-MWCNTs with BSA was investigated.
• The binding site of HO-MWCNTs on BSA was near to domain II and domain I of BSA.
• HO-MWCNTs acted as a pusher to increase the rate of fibrillation of BSA.
• The ligand binding and unfolding of BSA were also affected by HO-MWCNTs.

In order to understand the effects of carbon nanotubes on the structural stability of proteins, the ligand-binding ability, fibrillation, and chemical denaturation of bovine serum albumin in the presence of a multi-walled hydroxylated carbon nanotubes (HO-MWCNTs) was characterized by UV–vis, circular dichroism, fluorescence spectroscopy and molecule modeling methods at the molecular level. The experiment results indicated that the fluorescence intensity of BSA was decreased obviously in presence of HO-MWCNTs. The binding interaction of HO-MWCNTs with BSA led to the secondary structure changes of BSA. This interaction could not only affect the ligand-binding ability of BSA, but also change the rate of fibrillation and denaturation of BSA. This work gave us some important information about the structures and properties of protein induced by carbon nanotubes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 124, 24 April 2014, Pages 556–563
نویسندگان
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