Distinction of leukemia patients’ and healthy persons’ serum using FTIR spectroscopy

In this paper, FTIR spectroscopy was applied to compare the serum from leukemia patients with the serum from healthy persons. IR spectra of leukemia patients’ serum were similar with IR spectra of healthy persons’ serum, and they were all made up of proteins, lipids and nucleic acids, etc. In order to identify leukemia patients’ serum and healthy persons’ serum, the H1075/H1542, H1045/H1467, H2959/H2931 ratios were measured. The H2959/H2931 ratio had the highest significant difference among these ratios and might be a useful factor for identifying leukemia patients’ serum and healthy persons’ serum. Furthermore, from curve fitting, the RNA/DNA (A1115/A1028) ratios were observed to be lower in leukemia patients’ serum than those in healthy persons’ serum. The results indicated FTIR spectroscopic study of serum might be a useful tool in the field of leukemia research and diagnosis.

FTIR spectroscopy was used to distinguish leukemia patients’ serum (A) from healthy persons’ serum (B).Figure optionsDownload as PowerPoint slideHighlights
► Leukemia patients’ and healthy persons’ serum was studied by FTIR spectroscopy.
► IR spectra of serum were investigated.
► H1075/H1542, H1045/H1467, H2959/H2931 ratios of the above samples were compared.
► Curve fitting was used to study differences of the above serum.
► FTIR spectroscopy can indentify leukemia patients’ and healthy persons’ serum.