Spectrometry researches on interaction and sonodynamic damage of riboflavin (RF) to bovine serum albumin (BSA)

In this paper, the riboflavin (RF) was used to study the interaction and sonodynamic damage to bovine serum albumin (BSA) by fluorescence and UV–vis spectroscopy. The results showed that the RF could efficiently bind to BSA in aqueous solution. Under ultrasonic irradiation, the RF could obviously damage the BSA. In addition, synchronous fluorescence spectroscopy revealed that the RF showed more accessible to tryptophan (Trp) residues than to tyrosine (Tyr) residues. Also, it damaged Trp residues more seriously than Tyr residues under ultrasonic irradiation. At last, the generation of reactive oxygen species (ROS) in sonodynamic process was estimated by the method of Oxidation–Extraction Spectrometry (OES). And then, several radical scavengers were used to determine the kind of ROS. It was found that at least the singlet oxygen (1O2) and hydroxyl radicals (OH) were generated.

The riboflavin (RF) is used as sonosensitive compound to study the interaction and sonodynamic damage to bovine serum albumin (BSA) by fluorescence and UV–vis spectroscopy. In aqueous solution the RF can bind to BSA molecules efficiently. The synergetic effect of RF and ultrasonic irradiation can obviously damage the BSA molecules. Synchronous fluorescence spectroscopy revealed that the RF displays a more accessibility to tryptophan (Trp) residues than to tyrosine (Tyr) residues. Also, the tryptophan (Trp) residues are damaged more seriously than tyrosine (Tyr) residues under ultrasonic irradiation. The generation of ROS in sonodynamic process is estimated, and then, the kind of ROS was determined. It was found that at least the singlet oxygen (1O2) and hydroxyl radicals (OH) were generated.Figure optionsDownload as PowerPoint slideHighlights
► The interaction of riboflavin (RF) with BSA was studied.
► The sonodynamic damage of BSA in the presence of RF was studied.
► The formations of ROS in sonocatalytic process were studied.
► The kind of generated ROS were determined by adding some radical scavengers.