کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1236210 968864 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly sensitive spectrofluorimetric determination of trace amounts NADP using Europium ion–doxycycline complex probe
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Highly sensitive spectrofluorimetric determination of trace amounts NADP using Europium ion–doxycycline complex probe
چکیده انگلیسی

A new spectrofluorimetric method was developed for determination of trace amount of Coenzyme II (NADP). Using europium ion-doxycycline (DC) as a fluorescent probe, in the buffer solution of pH 8.44, NADP can remarkably enhance the fluorescence intensity of the Eu3+–DC complex at λ = 612 nm and the enhanced fluorescence intensity is in proportion to the concentration of NADP. Optimum conditions for the determination of NADP were also investigated. The dynamic range for the determination of NADP is 3.3 × 10−7 to 6.1 × 10−6 mol l−1 with detection limit of 6.8 × 10−8 mol l−1. This method is simple, practical and relatively free interference from coexisting substances and can be successfully applied to determination of NADP in synthetic water samples and in serum samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the Eu3+–DC system and the Eu3+–DC–NADP system have been also discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 65, Issue 1, September 2006, Pages 62–66
نویسندگان
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