Fluorescence enhancement of yttrium(III)-rutin by nucleic acids in the presence of cetyltrimethylammonium bromide

It is found that nucleic acids can enhance the fluorescence intensity of yttrium(III) (Y3+)-rutin in presence of cetyltrimethylammonium bromide (CTMAB) system. In hexamethylenetetramine (HMTA)-HCl buffer, the maximum enhanced fluorescence is produced, with maximum excitation and emission wavelengths at 452 and 520 nm, respectively. Based on this, a new fluorimetric method of determination of nucleic acids is proposed. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 1.0 × 10−7 to 1.0 × 10−5 g/ml for fish sperm DNA (fsDNA), 1.0 × 10−7 to 4.6 × 10−6 g/ml for yeast RNA (yRNA), their detection limits (S/N = 3) are 7.5 × 10−8, 8.0 × 10−8 g/ml, respectively. The interaction mechanism is also studied.