کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1236336 | 1495288 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Study on the interaction of copper–zinc superoxide dismutase with aluminum ions by electrochemical and fluorescent method Study on the interaction of copper–zinc superoxide dismutase with aluminum ions by electrochemical and fluorescent method](/preview/png/1236336.png)
The interaction of superoxide dismutase (SOD) with aluminum (Al) ions was investigated by cyclic voltammetry, fluorescence spectroscopy and synchronous fluorescence spectroscopy. The electrochemical activity of the SOD enzyme electrode was inhibited irreversibly by the addition of Al. Meanwhile, the static fluorescence quenching mechanism further revealed the existing of molecular complex of SOD with Al3+. The association constant was obtained from Lineweaver-Burk plot. The experimental results of voltammetry and fluorescence spectroscopy indicated that the conformation of SOD molecule was altered by the formation of Al–SOD complex. It may influence the activity of SOD enzyme since the optimum action of SOD depends upon a particular configuration of electrostatic charges in the enzyme molecule.
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 65, Issues 3–4, November 2006, Pages 896–900