کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1236417 | 968869 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering](/preview/png/1236417.png)
چکیده انگلیسی
A simple, sensitive and selective method was proposed for the determination of proteins by using a resonance light scattering technique. The weak resonance light scattering (RLS) of Bordeaux red (BR) can be enhanced greatly in the pH range 3.87-3.96 by the addition of micro amounts of proteins, resulting in four characteristic peaks in the wavelength range 250-600 nm. At the maximal wavelength of 363 nm, the enhanced RLS is proportional to the concentration of proteins in the range 0.12-10.8 μg mlâ1 for bovine serum albumin (BSA) and 0.24-18.0 μg mlâ1 for human serum albumin (HSA). The detection limits were 40.0 and 52.9 ng mlâ1 for BSA and HSA, respectively. The present method has been applied to the determination of total proteins in human serum, urine and saliva samples. The obtained results are in good agreement with those obtained by the Bradford method with relative standard deviations (R.S.D.) of 0.9-2.3%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 64, Issue 3, June 2006, Pages 574-579
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 64, Issue 3, June 2006, Pages 574-579
نویسندگان
Suling Feng, Zihong Pan, Jing Fan,