کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1236434 | 968869 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of nucleic acids with a near infrared cyanine dye using resonance light scattering technique
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of nucleic acids with a near infrared cyanine dye using resonance light scattering technique Determination of nucleic acids with a near infrared cyanine dye using resonance light scattering technique](/preview/png/1236434.png)
چکیده انگلیسی
A new method for the determination of nucleic acids has been developed based on the enhancement effect of resonance light scattering (RLS) with a cationic near infrared (NIR) cyanine dye. Under the optimal conditions, the enhanced RLS intensity at 823 nm is proportional to the concentration of nucleic acids in the range of 0-400 ng mLâ1 for both calf thymus DNA (CT DNA) and fish sperm DNA (FS DNA), 0-600 ng mLâ1 for snake ovum RNA (SO RNA). The detection limits are 3.5 ng mLâ1, 3.4 ng mLâ1 and 2.9 ng mLâ1 for CT DNA, FS DNA and SO RNA, respectively. Owing to performing in near infrared region, this method not only has high sensitivity endowed by RLS technique but also avoids possible spectral interference from background. It has been applied to the determination of nucleic acids in synthetic and real samples and satisfactory results were obtained.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 64, Issue 3, June 2006, Pages 698-702
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 64, Issue 3, June 2006, Pages 698-702
نویسندگان
Fang Fang, Hong Zheng, Ling Li, Yuqin Wu, Jinlong Chen, Shujuan Zhuo, Changqing Zhu,