کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1236758 | 968875 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study on the interaction between hematoporphyrin monomethyl ether and DNA and the determination of hematoporphyrin monomethyl ether using the resonance light scattering technique
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کلمات کلیدی
PDTCtDNARLSHMME3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromideCalf thymus DNA - DNA هویج هویجMTT - MTTInteraction - اثر متقابلBinding constant - ثابت اتصالPhotodynamic therapy - درمان فتودینامیکGemini surfactant - سورفکتانت GeminiBinding sites - محل های اتصالHematoporphyrin monomethyl ether - هماتوپورفیرین مونو متیل اترResonance light scattering - پراکندگی نور رزونانسResonance light scattering (RLS) - پراکندگی نور رزونانس (RLS)
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The interaction between photosensitizer anticancer drug hematoporphyrin monomethyl ether (HMME) and ctDNA has been studied based on the decreased resonance light scattering (RLS) phenomenon. The RLS, UV-vis and fluorescence spectra characteristics of the HMME-ctDNA system were investigated. Besides, the phosphodiesters quaternary ammonium salt (PQAS), a kind of new gemini surfactant synthesized recently, was used to determine anticancer drug HMME based on the increasing RLS intensity. Under the optimum assay conditions, the enhanced RLS intensity was proportional to the concentration of HMME. The linear range was 0.8-8.4 μg mLâ1, with correlation coefficient R2 = 0.9913. The detection limit was 0.014 μg mLâ1. The human serum samples and urine samples were determined satisfactorily, which proved that this method was reliable and applicable in the determination of HMME in body fluid. The presented method was simple, sensitive and straightforward and could be a significant method in clinical analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 77, Issue 3, 15 October 2010, Pages 605-611
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 77, Issue 3, 15 October 2010, Pages 605-611
نویسندگان
Zhanguang Chen, Tianhe Song, Xi Chen, Shaobin Wang, Junhui Chen,