کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1236857 | 968878 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study on the interaction among molecules and the determination of DNA by light scattering with a new type rhodanine derivative
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this paper the resonance Rayleigh scattering (RRS) and second-order scattering (SOS) spectrums of interaction between deoxyribonucleic acid (DNA) and 3-(4â²-methylphenyl)-5-(2â²-sulfophenylazo) rhodanine (4MRASP) in the environment of surface active substance sodium dodecyl sulfate (SDS) were reported. The light scattering intensity of 4MRASP was enhanced obviously and directly proportional to the concentrations of DNA when DNA was present. Based on this, two new determination methods of DNA were established with high sensitivity and selectivity, and the limits of detection were 8.00 and 1.07 ng mLâ1. They were applied to the determination of trace amounts of nucleic acid in synthetic and practical samples with satisfactory results. At last, the active mechanism among molecules was studied by ultraviolet spectrum, scattering spectrum and thermodynamics, which showed that the active force was changed from hydrophobic force to electrostatic force before and after SDS was added, and the mechanism of sensitization effect of SDS was proposed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 74, Issue 4, November 2009, Pages 875-880
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 74, Issue 4, November 2009, Pages 875-880
نویسندگان
Jinghua Yu, Bo Li, Ping Dai, Shenguang Ge, Xiaoliang Cheng,