کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1238906 968965 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects
چکیده انگلیسی

We report methods for correcting the photoluminescence emission and excitation spectra of highly absorbing samples for re-absorption and inner filter effects. We derive the general form of the correction, and investigate various methods for determining the parameters. Additionally, the correction methods are tested with highly absorbing fluorescein and melanin (broadband absorption) solutions; the expected linear relationships between absorption and emission are recovered upon application of the correction, indicating that the methods are valid. These procedures allow accurate quantitative analysis of the emission of low quantum yield samples (such as melanin) at concentrations where absorption is significant.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 61, Issue 9, July 2005, Pages 2153–2160
نویسندگان
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