Capillary electrophoresis with laser-induced fluorescence detection of main polyamines and precursor amino acids in saliva

A hollow-fiber liquid-phase microextraction (HF-LPME) method has been developed for the purification and preconcentration of biogenic polyamines and their precursor amino acids in human saliva. Putrescine (Put), cadaverine (Cad), spermidine (Spe), ornithine (Orn), lysine (Lys), and arginine (Arg) were determined by the CE-LIF detection after microextraction. Several factors that affect extraction efficiency, separation, and detection were investigated. Under the optimum conditions, six analytes could achieve baseline separation within 30 min, exhibiting a linear calibration at three orders of magnitude (r2 > 0.998); the obtained enrichment factors of HF-LPME were between 19 (for Orn) and 218 (for Cad), and the LODs were in the range of 0.0072–0.26 nmol/L. The proposed HF-LPME/CE-LIF method has been successfully applied for the sensitive analyses of the real-world saliva samples collected from healthy volunteers and different patients with oral diseases, providing a potential method for primary non-invasive diagnosis of some oral diseases.

A capillary electrophoresis with a laser-induced fluorescence detection (CE-LIF) method has been developed for simultaneous determination of three main polyamines (putrescine, cadaverine and spermidine), and their precursor amino acids (ornithine, lysine and arginine) in human saliva. And a hollow-fiber liquid-phase microextraction (HF-LPME) technology was applied for the purification and preconcentration of the targeted analytes, which significantly improved the LODs of the proposed method.Figure optionsDownload as PowerPoint slide