Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) matrices for agricultural application in crops

The vitamin C (VC) in crops was successfully determined using ascorbate oxidase (AO) electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) (PEDOT) matrices, which was easily prepared by one-step electrodeposition technique in ionic liquid microemulsions. The fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC, wide linear range, low detection limit, fast response time, good operational and storage stability, the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.