Rapid separation of acetophenone and its monohydroxy isomers by capillary electrophoresis

This work describes the development of a capillary electrophoresis (CE) method for the simultaneous separation of acetophenone (AP), 2-hydroxyacetophenone (2-HAP), 3-hydroxyacetophenone (3-HAP) and 4-hydroxyacetophenone (4-HAP) in synthetic mixtures using 10 mmol/L of sodium tetraborate buffer (pH 9.5). The aim of this work is to demonstrate the effectiveness of CE to separate AP and its monohydroxy isomers and to define how the separations are affected by buffers, buffer pH, sample matrices and separation voltage. This method was successfully used for the trace level separation and determination of 2-HAP, 3-HAP and 4-HAP in synthetic mixture and 4-HAP in spiked plasma samples.

This work describes the development of a capillary electrophoresis method for the simultaneous separation of acetophenone (AP), 2-hydroxyacetophenone (2-HAP), 3-hydroxyacetophenone (3-HAP) and 4-hydroxyacetophenone (4-HAP) in synthetic mixtures using 10 mmol/L of sodium tetraborate buffer (pH 9.5). The developed method was successfully applied to the analysis of 4-HAP in spiked plasma samples.Figure optionsDownload as PowerPoint slide