A novel fluorescent detection for PDGF-BB based on dsDNA-templated copper nanoparticles

A novel method for the detection of PDGF-BB has been developed using double-strand DNA-copper nanoparticles (dsDNA-CuNPs) as fluorescent markers. This assay relies on the premise that the aptamer-based probe undergoes a conformational change upon binding with target protein, and subsequently triggers polymerization reaction to generate dsDNA. Then, the resultant dsDNA can be used as a template for the formation of CuNPs with high fluorescence. Under the optimized conditions, the proposed assay allowed sensitive and selective detection of PDGF-BB with a detection limit of 4 nmol/L. This possibly makes it an attractive platform for the detection of a variety of biomolecules whose aptamers undergo similar conformational change.

This assay mainly relies on that the aptamer-based probe undergoing a conformational transition upon binding with target protein, which subsequently triggers the polymerization reaction to generate double strand DNA. Then, the double strand DNA can act as an efficient template for the formation of copper nanoparticles with high fluorescence.Figure optionsDownload as PowerPoint slide