Investigation of the interaction of carbofuran and DNA by voltammetry and fluorescence with the aid of chemometrics

The interactions of carbofuran and DNA were studied using voltammetry and fluorescence spectroscopy. The formation of carbofuran–DNA makes the current peak of DNA decreased by voltammetry method. The binding number (n) and constant (Ka) for complex carbofuran–DNA were calculated to be 1.06 ± 0.04 and 0.11 ± 0.03 mol−1 L, respectively by fluorescence measurement. Chemometrics approach, such as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug–DNA binding process. And the pure spectra and concentration profiles in the kinetic system were clearly deduced by multivariate curve resolution alternating least squares (MCR-ALS) with the initial estimates by evolving factor analysis (EFA).