High performance liquid chromatographic separation of clausenamide enantiomers with chiral-AGP stationary phase

A method of high performance liquid chromatographic separation of clausenamide enantiomers with chiral-AGP (α1-acid glycoprotein) stationary phases has been established. The absolute configurations of (−)clausenamide and (+)clausenamide are 3S, 4R, 5R, 6S and 3R, 4S, 5S, 6R, respectively. The present method has been used to analyze the (−)clausenamide and (+)clausenamide and its analogues such as the major metabolite and synthetic derivatives of clausenamide.