Differential pulse voltammetric studies on the effects of Al(III) on the lactate dehydrogenase activity

In this paper, differential pulse voltammetry (DPV) was applied to study the effects of aluminum Al(III) on the lactate dehydrogenase (LDH) activity. Michaelis–Menten constant (KmNADH) and maximum velocity (vmaxvmax) in the enzyme promoting catalytic reaction of “pyruvate (Pyr)+NADH+H+⇌LDHlactate+NAD+” under different conditions by monitoring DPV reduction current of NAD+ were reported.