Gas chromatography–mass spectrometry analysis and total phenolic contents of various crude extracts from the fruits of Datura metel L.

Datura metel is considered as medicinal plants worldwide. Locally it is used as a traditional medicine due to its medicinal values. The objective of this present work is to identify the chemical ingredients and evaluation of total phenolics contents of different crude extracts from the locally grown D. metel. In vitro evaluation of total phenolics contents of different crude extracts was measured by established Folin–Ciocalteu reagents method. The identification of chemical ingredients was carried out in various crude extracts of D. metel by using sensitive gas chromatography–mass spectrometry (GC–MS). The results showed from different chromatogram that high percentage ingredients are high molecular weight ingredients such as polyphenolics and their derivatives, flavonoids, triterpeniods, hydrocarbons etc. The identified high percentage chemical ingredients present in the crude extracts are biologically active. The phenolic contents of crude extracts as gallic acid equivalents were found to be highest in chloroform followed by the order methanol > butanol > ethyl acetate > hexane extract. The identification of good number of chemical ingredients by spectrometry method in various crude extracts of D. metel fruits might have some ecological significance. In conclusion, chloroform crude extract contains highest phenolics content could be used as antibiotics.