کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
12622 805 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes
چکیده انگلیسی

In this paper we report on the metabolic response of human hepatocytes grown on polyethersulfone membranes surface modified with a plasma-deposited acrylic acid coating and RGD peptide covalently immobilized through a “spacer arm” molecule. The modified surfaces were characterized by means of X-ray photoelectron spectroscopy and water contact angle measurements.The performance of modified and unmodified membranes was evaluated by assessing the expression of liver specific and biotransformation functions of human hepatocytes. Diclofenac, a non-steroidal anti-inflammatory drug, was used to investigate the biotransformation functions.Surface-modified membranes elicit specific cellular responses and induce hepatocytes to enhance the synthesis rate of albumin and urea, particularly in the presence of diclofenac. Also the biotransformation functions were expressed at high levels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 26, Issue 21, July 2005, Pages 4432–4441
نویسندگان
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