کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1268732 972418 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct electron transfer and electrocatalysis of hemoglobin in ZnO coated multiwalled carbon nanotubes and Nafion composite matrix
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Direct electron transfer and electrocatalysis of hemoglobin in ZnO coated multiwalled carbon nanotubes and Nafion composite matrix
چکیده انگلیسی

ZnO coated multiwalled carbon nanotubes (ZnO-MWCNTs) nano-composite was synthesized by the hydrothermal method and expected to offer a promising template for biosensor fabrication due to satisfying biocompatibility and improved properties. The ZnO-MWCNTs nano-composite was mixed with Nafion solution to form a composite matrix for the fabrication of hemoglobin (Hb) biosensor. This composite matrix combined the advantages of inorganic composite (ZnO-MWCNTs) and organic polymer (Nafion) and could promote the direct electron transfer of Hb. The Hb/ZnO-MWCNTs/Nafion film exhibited a pair of well-defined, quasi-reversible redox peaks with a formal potential of − 0.353 V (vs. SCE), characteristic of heme redox couple (Fe(III)/Fe(II)) of Hb. Hb retained its near-native conformation in the composite film. The immobilized Hb showed fast and excellent electrocatalytic activity to H2O2 with a linear range from 2 × 10− 7 to 1.2 × 10− 5 M, and the detection limit was 8.4 × 10− 8 M. The sensitivity and apparent Michaelis–Menten constant were 1.31 A/(M·cm2) and 82.8 μM, respectively, which indicated that Hb had a high affinity to H2O2. This biosensor also showed excellent electrocatalytic activity towards trichloroacetic acid.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 78, Issue 2, June 2010, Pages 106–112
نویسندگان
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