کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1271573 1496922 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amperometric immunobiosensor for α-fetoprotein using Au nanoparticles/chitosan/TiO2–graphene composite based platform
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Amperometric immunobiosensor for α-fetoprotein using Au nanoparticles/chitosan/TiO2–graphene composite based platform
چکیده انگلیسی

A simple label-free amperometric immunosensor for protein detection is developed based on TiO2–graphene (TiO2–Gr), chitosan and gold nanoparticles (AuNPs) composite film modified glassy carbon electrode (GCE). The negatively charged AuNPs can be adsorbed on the positively charged chitosan/TiO2–Gr composite film by electrostatic adsorption, and then is used to immobilize α-fetoprotein antibody for the assay of α-fetoprotein (AFP). The interaction of antigen and antibody on the electrode interface makes a barrier for electrons and inhibits the electro-transfer, resulting in the decreased DPV signals of probe Fe(CN)63 −/4 −. Using this strategy, a wide detection range (0.1–300 ng mL− 1) with the correlation coefficients of 0.992–0.994 for model target AFP is obtained. The limit of detection is 0.03 ng mL− 1 at a signal-to-noise ratio of 3. The results prove that the sensing strategy possesses sensitivity, selectivity, stability and generality, and it may be used to immobilize other biomoleculars to develop biosensor for the detection of other antigens or biocompounds.


► An immunosensor based on TiO2–graphene/chitosan/AuNPs composite film is developed.
► Detection range of 0.1–300 ng mL− 1 and detection limit of 0.03 ng mL− 1 for α-fetoprotein is obtained.
► Human serum samples were determined with recoveries of 92.6–108.1%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 90, April 2013, Pages 18–23
نویسندگان
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