کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1272283 972554 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of selected drugs in human urine by differential pulse voltammetry technique
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Determination of selected drugs in human urine by differential pulse voltammetry technique
چکیده انگلیسی

A new, simple and selective differential pulse voltammetry (DPV) method for the simultaneous determination of selected drugs in model solutions and spiked human urine samples with prior extraction was developed and validated. The objects of analysis were paracetamol, furosemide, dipyrone, cefazolin and dexamethasone belonging to four different therapeutic groups (antibiotics, analgesic, demulcent and diuretic). Analytical methods for the preparation of urine samples for voltammetric analysis (liquid–liquid extraction — LLE and solid-phase extraction — SPE) were worked out and optimized. Hanging mercury drop electrode (HMDE) and graphite electrode were used as working electrodes. Reference electrode was Ag|AgCl|KCl(sat.), whereas auxiliary electrode — platinum electrode. The optimal conditions for quantitative determination were obtained in a Britton–Robinson (BR) buffer at pH 2.4. Quantification was performed by means of calibration curve and standard addition methods. The calibration curves of analysed drugs are linear within the range of concentration: 6.61–66.10, 6.05–54.42, 6.00–65.00, 4.20–33.58 and 0.51–3.06 μM for paracetamol, furosemide, dipyrone, cefazolin and dexamethasone, respectively. The levels of analysed compounds in human urine can be successfully determined using this developed method with no matrix effect.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 73, Issue 1, June 2008, Pages 5–10
نویسندگان
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