Electrochemical detection of DNA hybridization using metallacarborane unit

The evaluation of novel electrochemically active label for electrochemical detection of DNA hybridization is presented. Metallacarborane units modified with iron, cobalt or chromium were investigated. The value of redox potential and relatively strong current signal facilitate usage of Fe-carborane as marker covalently attached to the ssDNA. In electrochemical genosensor the sequence complementary to UL55 gene was labeled and used as a target for biosensor device. Interactions were investigated using electrochemical and piezoelectric methods. Obtained results confirm usefulness of the designed label in electrochemical detection of DNA hybridization.

► Complexes of carboranes with Co3 +, Cr3 + and Fe3 + metal cations were tested as electrochemical markers for DNA biosensors.
► Electrochemical signal can be observed only with the metallacarborane marker located away from the electrode.
► Fe-carboranes can be applied as useful markers for electrochemical DNA sensing.