DNA interaction and in-vitro antibacterial studies of fluoroquinolone based platinum(II) complexes

A series of square planar complexes of the type [Pt(FQ)2] (where FQ is deprotonated fluoroquinolone family drug) has been synthesized and characterized. The synthesized complexes have been checked for their in-vitro antimicrobial activity against two Gram(+ ve) and three Gram(− ve) bacterial species. Intrinsic binding constant (Kb) and mode of binding of complexes toward Herring Sperm DNA (HS DNA) have been determined using absorption titration and viscosity measurement, suggesting the partial intercalative mode of binding. Cleavage ability of complexes has been checked using gel electrophoresis technique and the result shows that the synthesized complexes cleave the DNA more efficiently compared to their respective fluoroquinolones and metal salt.

The bulkiness and non-planarity of the fluoroquinolones affect the Kb values. The complexes binds to DNA via partial intercalative mode of binding. Absorption titration and viscosity measurement supports the same binding mode. The DNA cleavage efficiency of the complexes are in competition with metal salt and fluoroquinolones.Figure optionsDownload as PowerPoint slideHighlights
► Synthesis of Pt(II) complexes with fluoroquinolones.
► Characterization by elemental analysis, IR, UV–visible, TGA, mass spectrometry.
► All Pt(II) complexes have been tested for their antibacterial studies.
► The interaction of complexes with DNA has been performed.