کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1334953 | 979487 | 2009 | 9 صفحه PDF | دانلود رایگان |
A set of copper(II) complexes of glycine and methylated glycine derivatives, Cu(aa)2, consisting of C-dimethylglycine, l-alanine, N-dimethylglycine and sarcosine, was investigated for their DNA binding and nucleolytic properties by means of EPR and visible spectroscopy, and electrophoresis. They bind weakly to DNA with apparent binding constants in the range 1.8–2.9 × 103 M−1 with very similar orientation. No DNA cleavage is observed in the absence of exogenous agents. Copper(II) complexes of N-methylated derivatives bind to DNA more stereo-specifically and less strongly, and their oxidative DNA cleavage is less efficient than those of the corresponding C-methylated derivatives in the presence of hydrogen peroxide (H2O2) alone, or sodium ascorbate (NaHA) alone or tandem H2O2–NaHA. The oxidative DNA cleavage mechanism in the three systems involves a common copper(I) species. Neocuproine can inhibit DNA cleavage by these complexes.
DNA interaction and oxidative cleavage by a series of copper(II) methylated glycine derivatives in the presence of hydrogen peroxide alone or ascorbate alone or both reagents are described. Comparison of nucleolytic efficiency and mechanism of cleavage are also discussed.Figure optionsDownload as PowerPoint slide
Journal: Polyhedron - Volume 28, Issue 11, 6 July 2009, Pages 2219–2227