Antioxidant activity of methimazole–copper(II) bioactive species and spectroscopic investigations on the mechanism of its interaction with Bovine Serum Albumin

Free radical-mediated oxidative stress has been implicated in numerous autoimmune disorders including Graves’ disease. Hyperthyroidism results in a marked increase in intracellular antioxidant enzymes including superoxide dismutase. The later activity is significantly increased in untreated Graves’ patients while treatment with methimazole results in normalization of the free radical and antioxidant activity indices. In this context, and considering the findings mentioned above, the aim of the present study was to evaluate unknown biological activities of methimazole and its methimazole–copper(II) complex investigating their superoxide scavenger power. Under the applied experimental conditions, methimazole did not show superoxide dismutase (SOD) activity while the copper complexes exhibited a strong superoxide radical scavenging capacity. Bearing in mind that the capacity of drugs to bind and/or interact with albumin is essential for their pharmacokinetic and pharmacodynamic properties, a complete investigation of the binding ability of both compounds by using Fourier transform infrared (FT-IR), Raman and Fluorescence spectroscopies, and UV–Vis spectrophotometry was included. Besides, in order to probe the copper ligand environment the EPR spectra of such compounds were analyzed.

Unknown biological activities of methimazole and its methimazole–copper(II) complex were explored and a complete investigation of the binding ability of both compounds to albumin was performed using Fourier transforms infrared (FT-IR), Raman and Fluorescence spectroscopies and UV–Vis spectrophotometry. To probe the copper ligand environment also their EPR spectra were measured.Figure optionsDownload as PowerPoint slideHighlights
► Biological activities of methimazole and its methimazole–copper(II).
► Investigation of the binding ability of both compounds to albumin was performed.
► In combination, FT-IR, Raman, EPR, Fluorescence and UV–Vis spectroscopies were applied.
► Identification of active solution specie: methimazole–copper(II)–BSA complex.