کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1363959 | 981525 | 2009 | 4 صفحه PDF | دانلود رایگان |
The synthesis of a series of conjugated spermine derivatives with benzoxadiazole, phenylxanthene or bodipy fluorophores is described. These fluorescent probes were used to identify the activity of the polyamine transport system (PTS). N1-Methylspermine NBD conjugate 5 proved to have the optimal fluorescence characteristics and was used to show a selectivity for PTS-proficient CHO versus PTS-deficient CHO-MG cells. It can therefore be used as a tool for the selection of cells sensitive to cytotoxic compounds vectored through the PTS.
N1-Methylspermine NBD 5 was synthesized from 4-chloro-7-nitrobenzo[1,2,5]oxadiazole (NBD) 2 and protected spermine 1. 5 was used as a fluorescent probe to select polyamine transport system (PTS) expressing cells, sensitive to cytotoxic compounds vectored through the PTS.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 19, Issue 9, 1 May 2009, Pages 2474–2477